![]() Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent. For example, a 1:10 dilution is a mixture of one part of a solution and nine parts fresh solvent. They are described as ratios of the initial and final concentrations. ![]() Serial dilutions are often performed in steps of 10 or 100. The initial concentration and target range needed determines the size and number of dilution steps required. Doing this several times results in a range of concentrations. The diluted sample is then used as the base solution to make an additional dilution. To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container, and additional water or other solvent * is added to dilute the original solution.
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